Metabolism of sucrose in a non-fermentative Escherichia coli under oxygen limitation
نویسندگان
چکیده
منابع مشابه
Effect of oxygen limitation and medium composition on Escherichia coli fermentation in shake-flask cultures.
Shake-flask cultures are widely used for screening of high producing strains. To select suitable strains for production scale, cultivation parameters should be applied that provide optimal growth conditions. A novel method of measuring respiratory activity in shake-flask cultures was employed to analyze Escherichia coli fermentation under laboratory conditions. Our results suggest that the leng...
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Although Escherichia coli strain EC3132 possesses a chromosomally encoded sucrose metabolic pathway, its growth on low sucrose concentrations (5 mM) is unusually slow, with a doubling time of 20 h. In this report we describe the subcloning and further characterization of the corresponding csc genes and adjacent genes. The csc regulon comprises three genes for a sucrose permease, a fructokinase,...
متن کاملRegulation of mutY and nature of mutator mutations in Escherichia coli populations under nutrient limitation.
Previous analysis of aerobic, glucose-limited continuous cultures of Escherichia coli revealed that G:C-to-T:A (G:C-->T:A) transversions were the most commonly occurring type of spontaneous mutation. One possible explanation for the preponderance of these mutations was that nutrient limitation repressed MutY-dependent DNA repair, resulting in increased proportions of G:C-->T:A transversions. Th...
متن کاملHexitol metabolism in Escherichia coli.
D-Sorbitol and dulcitol (galactitol), which, together with D-mannitol, are the three naturally occurring hexitols, have been known since the last quarter of the nineteenth century. Many fungi can utilize sorbitol for growth, but dulcitol is assimilated poorly by Aspergillus niger and a few yeasts. Among bacterial fermentations of these hexitols the best known are those by members of the Acetoba...
متن کاملGlycolate metabolism in Escherichia coli.
Hansen, Robert W. (University of Illinois College of Medicine, Chicago) and James A. Hayashi. Glycolate metabolism in Escherichia coli. J. Bacteriol. 83:679-687. 1962.-This study of glycolate-adapted Escherichia coli indicates that the most probable route for utilization of the substrate includes glyceric acid, 3-phosphoglyceric acid, and the tricarboxylic acid cycle. A glyceric acid dehydrogen...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2019
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-019-09909-6